A novel peridinin—chlorophyll a protein (PCP) from the marine dinoflagellate Alexandrium cohorticula: A high pigment content and plural spectral forms of peridinin and chlorophyll a

FEBS Letters

Kodama, Masaaki⁴  Nozawa, Tsunenori¹  Kobayashi, Masayuki¹  Nomura, Setsuzo⁴  Ogata, Takehiko⁴  Mimuro, Mamoru²  Katoh, Tetzuya³ 

[1] Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University, Aoba, Sendai 980-77, Japan;National Institute for Basic Biology, Myodaiji, Okazaki 444, Japan;Department of Botany, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan;School of Fisheries Sciences, Kitasato University, Sanriku-cho, Kesen-gun, Iwate 022-01, Japan

关键词: Carotenoid;    Dinoflagellate;    Peridinin;    Photosynthesis;    Pigment—protein complex;    Alexandrium cohorticula;    CD;    circular dichroism;    chl;    chlorophyll;    kDa;    kilodaltons;    MCD;    magnetic circular dichroism;    PCP;    peridinin—chlorophyll a protein;    PMSF;    phenylmethylsulfonylfluoride;    SDS-PAGE;    sodium dodecyl sulfate-poly acrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(94)01314-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new type of peridinin—chlorophyll a protein (PCP) was isolated from the marine dinoflagellate Alexandrium cohorticula. Unlike previous studies, PCP was obtained as a single component in the presence of a protease inhibitor. The monomer had a molecular mass of 37 kDa with 12 peridinin molecules associated with 2 chl a molecules. This pigment content was much higher than that reported previously. We observed a partial amino acid sequence of the N-terminus that is novel among photosynthetic pigment—protein complexes. Magnetic circular dichroism clearly indicated that chl a in PCP had monomeric features. Multiple spectral components were suggested for both chl a and peridinin. Based on the high pigment content, the optical properties were compared with those for a reported PCP containing 4 chl a and 1 peridinin.

【 授权许可】

Unknown