Regulation by glucose of the biosynthesis of PC2, PC3 and proinsulin in (ob/ob) mouse Islets of Langerhans

FEBS Letters

Benig, Michelle¹  Steiner, Donald F.¹  Carroll, Raymond¹  Martin, Sean K.¹ 

[1] Department of Biochemistry and Molecular Biology, The University of Chicago, and The Howard Hughes Medical Institute, 5841 S. Maryland Avenue, Chicago, IL 60637, USA

关键词: Prohormone convertase;    β-Cell;    Translational control;    Precursor processing;   

DOI  :  10.1016/0014-5793(94)01284-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The prohormone convertases PC2 and PC3 have been shown to catalyze the processing of proinsulin to insulin in pancreatic β-cells. In these studies we have compared the effects of glucose on PC2 and PC3 biosynthesis in freshly isolated islets from normal and hyperglycemic (ob/ob) mice. In contrast to normal islets [Alarcón, et al. (1993) J. Biol. Chem. 268, 4276] the biosynthesis of both PC2 and PC3 is stimulated by glucose, parallel to the stimulation of proinsulin in the (ob/ob) islets. Inhibition of PC2 biosynthesis by glucose in normal islet non β-cells may obscure stimulation of PC2 biosynthesis in normal islet β-cells.

【 授权许可】

Unknown