CD146: biosynthesis and production of a soluble form in human cultured endothelial cells

FEBS Letters

Combes, V¹  Dignat-George, F¹  Sampol, J¹  Bardin, N¹  Francès, V¹ 

[1] Laboratoire d'Hématologie et d'Immunologie, U.F.R. de Pharmacie, 27, Bd Jean Moulin, 13385 Marseille Cedex 5, France

关键词: Human endothelial cell;    Immunoglobulin superfamily;    Adhesion molecule;    Precursor processing;    Soluble CD146;   

DOI  :  10.1016/S0014-5793(97)01455-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously identified the S-Endo 1-associated antigen (CD146), an endothelial member of the immunoglobulin superfamily with a characteristic V-V-C2-C2-C2 Ig domain structure. In cultured human endothelial cells, we investigated its biosynthesis by immunoprecipitation and pulse-chase labeling. CD146 was synthesized as a 100 kDa precursor form, which was processed into a 120 kDa mature form. In the culture media of endothelial cells, we observed a CD146 soluble form that was about 10 kDa smaller than cell-associated CD146. In parallel with soluble forms of other members of the immunoglobulin superfamily, soluble CD146 could modulate and control the functions of the molecule.

【 授权许可】

Unknown