Is Zn2+ transported by the mitochondrial calcium uniporter?

FEBS Letters

Saris, Nils-Erik L.¹  Niva, Kaija¹ 

[1] The Helsinki Bioenergetics Group, POB 8, Department of Medical Chemistry, University of Helsinki, FIN-00014 Helsinki, Finland

关键词: Calcium;    Calcium uniporter;    Cyclosporin A;    Liver mitochondria;    Membrane potential;    Mitochondrial membrane permeabilisation;    TMPD;    N;    N;    N;    N-tetramethyl-1;    4-phenylenediaminedihydrochloride;    Tris;    tris-hydroxymethylaminomethane;   

DOI  :  10.1016/0014-5793(94)01256-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Zinc ions were found to inhibit Ca²⁺ uptake by rat liver mitochondria driven by succinate respiration but not that by a valinomycin-induced membrane potential. Zn²⁺ at 1 μM or higher concentrations induced a lowering of the membrane potential under the former but not the latter conditions. It is concluded that it is the lowered membrane potential in the presence of Zn²⁺ that reduces the rate of respiration-driven Ca²⁺. Ruthenium red was found to inhibit the uptake of Zn²⁺ but had no influence on its action upon the membrane potential. Zn²⁺ did not affect the Ruthenium red-insensitive Ca²⁺ efflux. Ca²⁺ stimulated the uptake of Zn²⁺. It is concluded that Zn²⁺ may be transported by the mitochondrial calcium uniporter but that it may have access to sites required for inhibition of respiration by other routes.

【 授权许可】

Unknown