Import of phosphatidylinositol and phosphatidylcholine into mitochondria of the yeast, Saccharomyces cerevisiae

FEBS Letters

Paltauf, Fritz¹  Lampl, Manfred¹  Daum, Günther¹  Leber, Andrea¹ 

[1] Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz, Petersgasse 12/2, A-8010 Graz, Austria

关键词: Phosphatidylinositol;    Phosphatidylcholine;    Phospholipid transfer;    Mitochondrion;    Membrane contact site;   

DOI  :  10.1016/0014-5793(94)01197-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An in vitro assay was designed to study the import of ³H-labeled phosphatidylinositol and phosphatidylcholine, respectively, from unilamellar vesicles into isolated mitochondria of the yeast, Saccharomyces cerevisiae. Both phospholipids reached the inner mitochondrial membrane. During import they were detected in contact sites between the outer and the inner mitochondrial membrane, supporting the notion that these zones are sites of intramitochondrial phospholipid transport. The uncoupler CCCP, the antibiotic adriamycin, and energy depletion caused by oligomycin and apyrase did not inhibit the transport of phosphatidylinositol and phosphatidylcholine into mitochondria.

【 授权许可】

Unknown