Cloning of the rat and human prostaglandin F2α receptors and the expression of the rat prostaglandin F2α receptor

FEBS Letters

Wahlqvist, J.²  Hellström-Lindahl, E.²  Gullberg, H.²  Lind, P.²  Stjernschantz, J.¹  Kinhult, A.²  Sjögren, A.-M.²  Lake, S.² 

[1] Pharmacia Opthalmics, Glaucoma Research, S-751 82 Uppsala, Sweden;Pharmacia BioScience Center, S-112 87 Stockholm, Sweden

关键词: Prostanoid receptor;    PGF2α;    Cloning;    Expression;    Ligand binding;    Northern analysis;   

DOI  :  10.1016/0014-5793(94)01198-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned the FP receptor from rat corpus luteum and human uterus cDNA libraries, respectively. The coding DNA sequence in the rat cDNA is 1101 bp and is similar to the mouse cDNA coding for a receptor protein of 366 amino acids. The human sequence shows a 5 bp deficiency in the 3′ region, truncating the coding sequence to 359 amino acids. Northern blot analysis indicates highest expression in the ovary. Cell lines have been established giving stable expression of the FP receptor. Activation of the cloned FP receptor gave an increase in intracellular calcium, indicating signaling via phospholipase C-mediated phosphoinositide turnover. Using [³H]PGF_2α, binding of PGs showed the rank order of fluprostenol > PhXA70 > PGF_2α > - PhXA85 > PGD₂ > PGE₂.

【 授权许可】

Unknown