Monoclonal antibodies against metalloporphyrins. Specificity of interaction with structurally different metalloporphyrins

FEBS Letters

Demcheva, Marina V.¹  Ponomarev, Gely V.¹  Mantrova, Ekaterina Yu.¹  Savitsky, Alexander P.¹ 

[1] A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, Moscow 117071, Russian Federation

关键词: Monoclonal antibody;    Metalloporphyrin;    Pd-coproporphyrin;    Hemoprotein;    Abzyme;   

DOI  :  10.1016/0014-5793(94)01221-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies against Pd-coproporphyrin I have been obtained. The antibody specificity for free as well as for conjugated Pd-coproporphyrin I is characterized. Affinity constants are estimated for 3 monoclonal antibodies effectively interacting with free Pd-coproporphyrin I. A comparative study on the binding of monoclonal antibodies with analogues and derivatives of Pd-coproporphyrin I has revealed that the antigen is mainly located inside the antibody paratope. The protein adjoins complementary to the metalloporphyrin in such a manner that antibodies obtained discern only isomer I, and to some degree, isomer III of coproporphyrin.

【 授权许可】

Unknown