Developmental regulation of gene expression for the MPTPδ isoforms in the central nervous system and the immune system

FEBS Letters

Katagiri, Tatsuo¹  Mizuno, Kazuya¹  Yakura, Hidetaka¹  Hasegawa, Kiminori¹  Ogimoto, Mami¹ 

[1] Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183, Japan

关键词: Protein tyrosine phosphatase;    Cell differentiation;    Isoform;    PTP;    protein tyrosine phosphatase;    PTK;    protein tyrosine;    kinase;    Ig;    immunoglobulin;    FN-III;    fibronectin type III repeat;    RT-PCR;    reverse transcription-polymerase chain reaction;    GAPDH;    glyceradehyde 3-phosphate dehydrogenase;   

DOI  :  10.1016/0014-5793(94)01188-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

MPTPδ is a murine transmembrane protein tyrosine phosphatase which has three isoforms, types A–C, differing in the structure of the extracellular regions. In this study, we examined MPTPδ isoform expression in the brain and the immune system at discrete developmental or differentiation stages. RT-PCR analysis demonstrated that another isoform, type D, is transcribed from the MPTPδ gene. In the brain, only type D was expressed until postnatal day 7 (P7), but after P14, all four isoforms were detected. In contrast, the spleen, thymus and all the hematopoietic cell lines examined express only types B and C isoforms. An in situ hybridization study showed that MPTPδ mRNA is diffusely expressed throughout the spleen, but its expression in the thymus is restricted to the medullary region.

【 授权许可】

Unknown