Ca2+‐ATPase distributes differently in cardiac sarcolemma than dihydropyridine receptor α1 subunit and Na+/Ca2+ exchanger

FEBS Letters

Takahashi, Masami²  Hanada, Hironori¹  Iwata, Yuko¹  Shigekawa, Munekazu¹ 

[1] Department of Molecular Physiology, National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan;Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan

关键词: Sarcolemmal Ca2+-ATPase;    Na+/Ca2+ exchanger;    DHP receptor α1 subunit Cardiac myocyte;    Hamster heart;    Canine heart;    SR;    sarcoplasmic reticulum;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide electrophoresis;    MBP;    maltose binding protein;   

DOI  :  10.1016/0014-5793(94)01174-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the distribution of the sarcolemmal Ca²⁺ transporters in hamster and dog ventricular myocytes by immunocytochemical and membrane fractionation techniques. The data suggest that the DHP receptor α1 subunit and the Na⁺/Ca²⁺ exchanger are present in surface sarcolemma as well as T-tubule membranes located at the cardiac dyads. Compared with these Ca²⁺ transporters, the sarcolemmal Ca²⁺-ATPase is much less abundant in the latter fraction. Thus the sarcolemmal Ca²⁺-ATPase seems to be located predominantly in surface sarcolemma.

【 授权许可】

Unknown