Heterologous expression of an active rat regulatory protein of glucokinase

FEBS Letters

Detheux, Michel¹  Van Schaftingen, Emile¹ 

[1] Laboratoire de Chimie Physiologique, Université Catholique de Louvain and International Institute of Cellular and Molecular Pathology, Avenue Hippocrate 75, B-1200 Brussels, Belgium

关键词: Glucokinase;    Glycolysis;    Fructose 1-phosphate;    Fructose 6-phosphate;   

DOI  :  10.1016/0014-5793(94)01160-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA presumed to encode the rat liver regulatory protein of glucokinase has been expressed in Escherichia coli and a partially soluble protein has been obtained. This recombinant protein was partially purified and found to have the same apparent molecular mass as the regulatory protein purified from rat liver. Like the latter, it inhibited rat liver glucokinase competitively with respect to glucose and its effect was sensitive to fructose 6-phosphate and fructose 1-phosphate.

【 授权许可】

Unknown