Sub‐compartmentation of the ‘cytosolic’ glucose 6‐phosphate pool in cultured rat hepatocytes

FEBS Letters

Christ, Bruno¹  Jungermann, Kurt¹ 

[1] Institut für Biochemie, Georg-August-Universität, Humboldtallee 23, D-3400 Göttingen, FRG

关键词: Glucose 6-phosphate;    Glucokinase;    Glycogenolysis;    Gluconeogenesis;    Glycolysis;    Filipin;   

DOI  :  10.1016/0014-5793(87)80959-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

[¹⁴C]Glucose release either from endogenous ¹⁴C-prelabelled glycogen or from added ¹⁴C-labelled glucose 6-phosphate was measured in filipin-treated, permeabilized hepatocytes in 48 h culture. [¹⁴C]Glucose output from prelabelled glycogen was not altered by the addition of 5 mM glucose 6-phospate to the incubation medium. Conversely, [¹⁴C]glucose release from 5 mM labelled glucose 6-phosphate was not influenced by different glycogen concentrations in the cells. Moreover, in the permeabilized cells the anion transport inhibitor DIDS (4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid) inhibited only the liberation of [¹⁴C]glucose from labelled glucose 6-phosphate but not from glycogen. It is therefore concluded that there exist at least 2 separate, mutually non-accessible glucose 6-phosphate pools in cultured rat hepatocytes, one linked to glycogenolysis and the other to gluconeogenesis.

【 授权许可】

Unknown