Thermal recovery of iodopsin from its meta I‐intermediate

FEBS Letters

Imamoto, Yasushi²  Yoshizawa, Tôru¹  Imai, Hiroo²  Shichida, Yoshinori² 

[1] Department of Information Systems Engineering, Faculty of Engineering, Osaka Sangyo University, Daito, Osaka 574, Japan;Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606-01, Japan

关键词: Cone visual pigment;    Iodopsin;    Rhodopsin;    Photobleaching process;    Isomerization;    Retinal protein;   

DOI  :  10.1016/0014-5793(94)01112-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thermal reaction of meta I-intermediate of iodopsin (metaiodopsin I), a chicken red-sensitive cone pigment, was studied by low-temperature spectrophotometry at −20°C. Irradiation of iodopsin at −20°C produced metaiodopsin I, whose absorption maximum was at about 470 nm. An incubation of metaiodopsin I at −20 °C resulted in a conversion to metaiodopsin II having absorption maximum at about 380 nm, as well as a concurrent formation of a red-shifted product stable at room temperature. Since the absorption spectrum and photo-reactivity of the red-shifted product were identical with those of iodopsin, the red-shifted product should be iodopsin. Thus a part of metaiodopsin I can revert to iodopsin by the thermal reaction unlike metarhodopsin I.

【 授权许可】

Unknown