Chicken red‐sensitive cone visual pigment retains a binding domain for transducin

FEBS Letters

Artamonov, Igor D.¹  Okano, Toshiyuki¹  Yoshizawa, Tôru²  Fukada, Yoshitaka¹ 

[1] Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan

关键词: Cone visual pigment;    Iodopsin;    GTP-binding protein;    Transducin;    Rhodopsin;    (Chicken retina);    GppNHp;    guanosine-5′-(β;    γ-imido)triphosphate;    DTT;    dithiothreitol;    PC;    fresh egg-yolk phosphatidylcholine;    Chaps;    (3-[(3-cholamidopropyl)dimethylammoniol-1-propanesulfonate);    PhMeSO2F;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(89)80255-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Iodopsin (a red-sensitive cone visual pigment) and rhodopsin (a rod pigment) were isolated from chicken retina. They were separately reconstituted into phosphatidylcholine liposomes and then mixed with rod transducin (Tα and Tβγ) purified from bovine retina. Iodopsin enhanced, only when irradiated, the binding of GppNHp to Tα to a similar extent to irradiated rhodopsin. Furthermore, the binding of GppNHp to Tα in the presence of a photobleaching intermediate of iodopsin preferably required Tβγ-2 rather than Tβγ-1, which is very similar in profile to that in the presence of the intermediate of rhodopsin (J. Biol. Chem., in press). These results indicate that the binding domain for transducin in iodopsin should closely resemble that in rhodopsin.

【 授权许可】

Unknown