RNA/DNA hybrid duplexes with identical nearest‐neighbor base‐pairs have identical stability

FEBS Letters

Nakano, Shu-ichi¹  Sasaki, Muneo¹  Ohmichi, Tatsuo¹  Katoh, Misa¹  Sugimoto, Naoki¹ 

[1] Department of Chemistry, Faculty of Science, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658, Japan

关键词: RNA/DNA hybrid;    Nearest-neighbor model;    Thermodynamic parameter;    Melting temperature;    Stability prediction;    Non-denaturing gel electrophoresis;    CD;    circular dichroism;    UV;    ultraviolet;    HPLC;    high-performance liquid chromatography;    T m;    melting temperature;    MOPS;    3-(N-morpholino)propanesulfonic acid;    Na2EDTA;    disodium ethylene-diaminetetraacetate;    Tris;    tris(hydroxymethyl)aminomethane;   

DOI  :  10.1016/0014-5793(94)01098-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy of these pairs was 4%, which was about expected within experimental error. These results indicate that the nearest-neighbor model is valid for predicting the stability of RNA/DNA hybrid duplexes as well as RNA/RNA and DNA/DNA duplexes.

【 授权许可】

Unknown