Genetic variations and dog breed identification using inter-simple sequence repeat markers coupled with high resolution melting analysis

PeerJ

article

Wannapimol Kriangwanich¹  Korakot Nganvongpanit¹  Kittisak Buddhachat²  Puntita Siengdee²  Siriwadee Chomdej²  Siriluck Ponsuksili⁴  Chatchote Thitaram⁶ 

[1] Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University;Excellence Center in Veterinary Bioscience, Chiang Mai University;Department of Biology, Faculty of Science, Naresuan University;Leibniz Institute for Farm Animal Biology;Department of Biology, Faculty of Science, Chiang Mai University;Center of Excellence in Elephant and Wildlife Research, Faculty of Veterinary Medicine, Chiang Mai University

关键词: Breeds;    Canine;    Classification;    Melting temperature;    Genetic variation;   

DOI  :  10.7717/peerj.10215

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The identification of differing physical characteristics of dogs is an uncomplicated and straightforward way to categorize dog breeds. However, many dog owners and veterinarians still struggle to distinguish between pure breed and mixed variations in certain breeds of dogs. Presently, the absence of the tools and methods needed to confirm a pure breed dog is a significant problem since the only method available to validate pure or mongrel breeds is the official pedigree system. Inter-simple sequence repeat markers have been successfully used to assess genetic variations and differentiations. Notably, inter-simple sequence repeat markers coupled with high resolution melting analysis were effectively used for the breed identification of 43 breeds of dogs (total 463 dogs). The 10 primers chosen for analysis resulted in a range of 31–78.6% of breed discrimination when using one primer, while a combination of two primers was able to successfully discriminate between all of the 43 dog breeds (100%). Shannon’s index information (I = 2.586 ± 0.034) and expected heterozygosity (He = 0.908 ± 0.003) indicated a high level of genetic diversity among breeds. The fixation index (Fst) revealed a value of 10.4%, demonstrating that there was a high level of genetic subdivision between populations. This study showed that inter-simple sequence repeat marker analysis was effective in demonstrating high genetic diversity among varying breeds of dogs, while a combination of Inter-simple sequence repeat marker analysis and high resolution melting analysis could provide an optional technique for researchers to effectively identify breeds through genetic variations.

【 授权许可】

CC BY