Multiple receptor‐like protein tyrosine phosphatases in the form of chondroitin sulfate proteoglycan

FEBS Letters

Hamanaka, Hiroki¹  Maeda, Nobuaki¹  Noda, Masaharu¹  Nishiwaki, Taeko¹  Shintani, Takafumi¹ 

[1] Division of Molecular Neurobiology, National Institute for Basic Biology, and Department of Molecular Biomechanics, The Graduate University for Advanced Studies, Okazaki 444, Japan

关键词: Protein tyrosine phosphatase;    Proteoglycan;    HNK-1 carbohydrate;    Rat brain;    PTPase;    protein tyrosine phosphatase;    MAb;    monoclonal antibody;    DTT;    dithiothreitol;    PMSF;    phenylmethylsulphonyl fluoride;    pNPP;    p-nitrophenyl phosphate;    PAGE;    polyacrylamide gel electrophoresis;    GST;    glutathione S-transferase;   

DOI  :  10.1016/0014-5793(94)01093-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possibility that some of the brain proteoglycans are receptor-like protein tyrosine phosphatases (PTPases) was investigated. Membrane-bound proteoglycan fractions were prepared from the postnuclear membrane fraction of 8-day-old rat brain by DEAE ion-exchange chromatography and CsCl density gradient centrifugation. The isolated proteoglycan fractions showed high PTPase specific activities together with the typical PTPase characteristics. Renaturation experiments indicated that chondroitin sulfate proteoglycans with 380- and 170-kDa core proteins carried the PTPase activity. The proteoglycan with 380-kDa core protein was identified as RPTPβ/ζ bearing HNK-1 carbohydrate.

【 授权许可】

Unknown