A new additive for protein crystallization

FEBS Letters

Cusack, Stephen³  Rabilloud, Thierry²  Vuillard, Laurent¹  Leberman, Reuben³  Berthet-Colominas, Carmen³ 

[1] Institut Laue-Langevin, BP 156, 38042 Grenoble Cedex 09, France;INSERM 309/DBMS BMCC CEN-G, 17 Rue des Martyrs, 38054 Grenoble Cedex 9, France;EMBL Grenoble Outstation, c/o ILL, BP 156, 38042 Grenoble Cedex 09, France

关键词: Crystallization;    Solubility;    NDSB;    non-detergent sulfobetaine;    NDSB195;    dimethyl ethylammonium propane sulfonate;   

DOI  :  10.1016/0014-5793(94)01060-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The potential usefulness of the new zwitterionic solubilizing agent, dimethyl ethylammonium propane sulfonate (NDSB195), in protein crystallization was shown using hen egg-white lysozyme. In the presence of this agent, highly diffracting crystals were obtained using ammonium sulphate as a precipitant, whereas in its absence only amorphous precipitates were obtained. The crystals possess a triclinic unit cell not previously described and diffract to a resolution of 2 Å. To ascertain that the new reagent had not produced significant changes in the protein fold the structure was determined to a resolution of 2.6 Å. Only minor differences were observed (notably in regions of crystal contacts) with the known tetragonal lysozyme structure (Brookhaven Protein Data Bank entry 1HEL).

【 授权许可】

Unknown