Collagen stimulates tyrosine phosphorylation of phospholipase C‐γ2 but not phospholipase C‐γ1 in human platelets

FEBS Letters

Watson, Steve P.²  Blake, Robert A.²  Schieven, Gary L.¹ 

[1] Department of Autoimmunity and Transplantation, Bristol Myers Squibb Pharmaceutical Research Institute, Seattle, WA 98121, USA;Department of Pharmacology, Mansfield Rd, Oxford OX1 3QT, UK

关键词: Collagen;    Fcγ receptor;    Wheat germ agglutinin;    Phospholipase Cγ;    Human platelet;    ARAM;    antigen recognition activation motif;    BAPTAAM;    acetomethoxy ester of 1;    2-bis(2-aminophenoxyethane)- N;    N;    N′;    N′-tetraacetic acid;    mAb;    monoclonal antibody;    PLC;    phosphoinositide-specific phospholipase C;    FcγRII;    the platelet low-affinity IgG receptor;    Fcγ receptor II;    SDS;    sodium dodecly sulfate;    PIP2;    phosphatidylinositol (4;    5)-biphosphate;    PKC;    protien kinase C;    SH;    src homology domain;    TxA2;    thromboxane A2;    PMSF;    phenylmethylsulfonyl flouride;    PDGF-β;    platelet-derived growth factor-β;   

DOI  :  10.1016/0014-5793(94)01037-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Collagen is an important primary stimulus of platelets during the process of hemostasis. As with many other platelet stimuli, collagen signal transduction involves the hydrolysis of inositol phospholipids; however, the mechanism which underlies this event is not well understood. Neither the collagen receptor nor the isoform of phospholipase C that is activated have been identified. We report that collagen-activation of platelets induces tyrosine phosphorylation of phospholipase C-γ2 but not phospholipase C-γ1. We also show that the platelet low affinity Fc receptor (FcγRII), which mediates activation of platelets by immune complexes, and wheat germ agglutinin, which binds non-specifically to glycoprotein, stimulate phospholipase C-γ2 tyrosine phosphorylation. In contrast, we could not detect phospholipase C-γ2 tyrosine phosphorylation in platelets stimulated by either thrombin or a stable thromboxane A₂ analogue, U46619.

【 授权许可】

Unknown