Yta10p, a member of a novel ATPase family in yeast, is essential for mitochondrial function

FEBS Letters

Feldmann, Horst²  Mannhaupt, Gertrud²  Langer, Thomas¹  Schnall, Ralf²  Tauer, Reimund²  Pajic, Alexander¹ 

[1] Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Goethestraße 33, D-80336 München, Germany;Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Schillerstraße 44, D-80336 München, Germany

关键词: Mitochondrion;    ATPase;    Yta family;    Saccharomyces cerevisiae;    Abfl;    ARS (autonomous replicating sequence) binding factor 1 from yeast;    Grf2;    general regulatory factor 2 from yeast;    PCR;    polymerase chain reaction;    PMSF;    phenylmethylsulfonylfluoride;    YTA;    genes encoding Yeast (human immunodeficiency virus) Tatbinding Analogues;   

DOI  :  10.1016/0014-5793(94)01045-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The yeast gene, YTA10, encodes a member of a novel family of putative ATPases. Yta10p, as deduced from the nucleotide sequence, is 761 amino acids in length (predicted molecular mass 84.5 kDa). The amino acid sequence of Yta10p exhibits high similarity to two other yeast proteins, Yta11 and Yta12, and to E coli FtsH. Several features of Yta10p are compatible with its localization in mitochondria. We report here that Yta10p is a yeast mitochondrial protein and that import is dependent on a membrane potential and accompanied by processing to a protein of approximately 73 kDa. Disruption of YTA10 leads to a nuclear petite phenotype and to a loss of respiratory competence, as shown by spectrophotometric measurement of the activities of respiratory complexes I–III and IV, respectively. These findings together with the high similarity of Yta10p to several ATP-dependent proteases suggest that Yta10p is a mitochondrial component involved, directly or indirectly, in the correct assembly and/or maintenance of active respiratory complexes.

【 授权许可】

Unknown