Molecular cloning and chromosomal localization of the human thrombopoietin gene

FEBS Letters

Sohma, Yoshiaki²  Kawamura, Kazuo²  Seki, Naohiko¹  Ogami, Kinya²  Hori, Tada-aki¹  Akahori, Hiromichi²  Kato, Takashi²  Miyazaki, Hiroshi²  Shimada, Yoshihiro² 

[1] Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263, Japan;Pharmaceutical Research Laboratory, Kirin Brewery Co. Ltd., 2-2 Soujamachi 1 Chome, Maebashi, Gunma 371, Japan

关键词: Thrombopoietin;    Genomic DNA;    Chromosome mapping;    TPO;    thrombopoietin;   

DOI  :  10.1016/0014-5793(94)01008-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

【 授权许可】

Unknown