Functional interaction between the epidermal growth factor receptor and c‐Src kinase activity

FEBS Letters

Ottenhoff-Kalff, Astrid E.¹  Staal, Gerard E.J.¹  Vendrig, Marijke P.¹  Oude Weernink, Paschal A.¹  Rijksen, Gert¹  van Beurden, Ellen A.C.M.¹ 

[1] Department of Hematology, Laboratory of Medical Enzymology, University Hospital Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands

关键词: c-Src;    Epidermal growth factor (EGF) receptor;    Breast cancer cell line;    EGF(-R);    epidermal growth fctor (-receptor);    PDGF-(-R);    platelet-derived growth factor (-receptor);    CSF-1;    colony-stimulating factor-1;    SH2;    Src homology 2;    PTK;    protein tyrosine kinase;    PVDF;    polyvinylidene diflouride;   

DOI  :  10.1016/0014-5793(94)00977-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To study the relationship between the tyrosine kinase c-Src and the epidermal growth factor receptor (EGF-R), we used the breast cancer cell line ZR75-1, which was transfected with the EGF-R. The EGF-R transfected cell line expressed 60 times more EGF-R than a control cell line transfected with the empty vector. In the presence of EGF, the EGF-R over-expressing cell line grew much faster than the control cell line. Both cell lines expressed approximately equal amounts of c-Src. However, the cell line over-expressing the EGF-R showed a twofold enhancement of c-Src kinase activity after EGF stimulation. The activation of c-Src kinase by EGF was confirmed in other EGF-R expressing cell types.

【 授权许可】

Unknown