FEBS Letters | |
Functional interaction between the epidermal growth factor receptor and c‐Src kinase activity | |
Ottenhoff-Kalff, Astrid E.1  Staal, Gerard E.J.1  Vendrig, Marijke P.1  Oude Weernink, Paschal A.1  Rijksen, Gert1  van Beurden, Ellen A.C.M.1  | |
[1] Department of Hematology, Laboratory of Medical Enzymology, University Hospital Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands | |
关键词: c-Src; Epidermal growth factor (EGF) receptor; Breast cancer cell line; EGF(-R); epidermal growth fctor (-receptor); PDGF-(-R); platelet-derived growth factor (-receptor); CSF-1; colony-stimulating factor-1; SH2; Src homology 2; PTK; protein tyrosine kinase; PVDF; polyvinylidene diflouride; | |
DOI : 10.1016/0014-5793(94)00977-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To study the relationship between the tyrosine kinase c-Src and the epidermal growth factor receptor (EGF-R), we used the breast cancer cell line ZR75-1, which was transfected with the EGF-R. The EGF-R transfected cell line expressed 60 times more EGF-R than a control cell line transfected with the empty vector. In the presence of EGF, the EGF-R over-expressing cell line grew much faster than the control cell line. Both cell lines expressed approximately equal amounts of c-Src. However, the cell line over-expressing the EGF-R showed a twofold enhancement of c-Src kinase activity after EGF stimulation. The activation of c-Src kinase by EGF was confirmed in other EGF-R expressing cell types.
【 授权许可】
Unknown
【 预 览 】
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