Cloning of a Xenopus laevis muscarinic receptor encoded by an intronless gene

FEBS Letters

Antonelli, Marcelo²  Olate, Juan¹  Carvallo, Pilar²  Herrera, Luisa¹ 

[1] Laboratorio Genética Molecular, Departamento de Fisiopatología, Facultad Ciencias Biológicas, Universidad de Concepción, Casilla 152-C, Concepción, Chile;Departamento de Bioquímica, Facultad de Medicina División Norte, Universidad de Chile, Casilla 70086, Santiago, Chile

关键词: G protein-coupled receptor;    cDNA cloning;    Xenopus laevis oocyte;   

DOI  :  10.1016/0014-5793(94)00957-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Xenopus laevis oocyte has endogenous sites that bind muscarinic agonists, which have been pharmacologically characterized as M3 and/or M1 receptor subtypes. In order to define the molecular identity of the receptor protein we have analyzed a Xenopus oocyte cDNA library and cloned a 2.9 kb cDNA fragment encoding a muscarinic receptor (xMR). The deduced amino acid sequence reveals a protein of 484 residues with an apparent molecular weight of 54,188 Da. Amino acid comparison with previously cloned mammalian muscarinic receptors showed a 78% identity with the human m4 subtype, presenting at the same time clustered differences within the amino-terminal region and third intracellular loop. Genomic Southern analysis displayed the presence of one main gene belonging to this subtype, and the PCR analysis revealed an intronless gene.

【 授权许可】

Unknown