Herbivore‐induced volatiles: The emission of acyclic homoterpenes from leaves of Phaseolus lunatus and Zea mays can be triggered by a β‐glucosidase and jasmonic acid

FEBS Letters

Boland, Wilhelm¹  Hopke, Jörn¹  Donath, Jens¹  Blechert, Siegfried² 

[1] Institut für Organische Chemie und Biochemie, Gerhard-Domagk-Str. 1, D-53121 Bonn, Germany;Institut für Organische Chemie der TU Berlin, Straße des 17. Juni 135, D-1000 Berlin, Germany

关键词: Volatile (mode of induction);    Elicitor;    Transducer;    β-Glucosidase;    Jasmonic acid;    Phaseolus lunatus;    Zea mays;   

DOI  :  10.1016/0014-5793(94)00948-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The treatment of healthy, undamaged plants of the Lima bean Phaseolus lunatus with solutions of a β-glucosidase from bitter almonds (at 5 U·ml⁻¹) through the petiole results in an enhanced emission of volatiles to the environment. The compounds are identical with those emitted in response to infestation with the red spotted spider mite Tetranychus urticae. Dominant products are the two acyclic homoterpenes 4,8-dimethyl-1,3E,7-dimethylnonatriene (homoterpene I) and 4,8,12-trimethyl-1,3E,7E,11-tridecatetraene (homoterpene II) which are of sesquiterpenoid and diterpenoid origin. Therefore, a β-glucosidase of the herbivore may be considered as the true elicitor for the odor induction. Homoterpene I and most other of the herbivore-induced volatiles can also be triggered by treatment of the plant with solutions of jasmonic acid (JA) at 100 nmol·ml⁻¹ to 10 μmol·ml⁻¹. The C₁₆ homoterpene II is not significantly induced by JA. The time—course of the enzymatic- and the JA-triggered induction of the volatiles is identical. The dose—response to JA parallels previous reports on alkaloid induction in cell cultures. In corn plants (Zea mays) JA triggers the emission of all volatiles which are known to be emitted in response to the damage by the beet army worm Spodoptora exigua. In summary, the emission of volatiles after damage by a herbivore resembles the production of phytoalexins in response to an attacking microorganism and uses similar elicitors and internal transduction pathways.

【 授权许可】

Unknown