Identification of metabolic pathways of the lipid peroxidation product 4‐hydroxynonenal by mitochondria isolated from rat kidney cortex

FEBS Letters

Ullrich, Oliver³  Siems, Werner G.¹  Esterbauer, Herrmann²  Henke, Wolfgang⁴  Grune, Tilman³ 

[1] Herzog-Julius-Hospital for Rheumatology and Orthopaedics, Kurhausstr. 13-17, D-38655 Bad Harzburg, Germany;Institute of Biochemistry, Karl-Franzens-University, Schubertstr. 1, A-8010 Graz, Austria;Clinics of Physical Therapy and Rehabilitation, Medical Faculty (Charite), Humboldt-University Berlin, Schumannstr. 20-21, D-10098 Berlin, Germany;Research Division of the Urological Clinic, Medical Faculty (Charite), Humboldt-University Berlin, Schumannstr. 20-21, D-10098 Berlin, Germany

关键词: Mitochondria;    4-Hydroxynonenal;    Aldehyde metabolism;    4-Hydrononenoic acid;    1;    4-Dihydroxynonene;    HNE-glutathione;    DHN;    1;    4-dihydroxynonene;    GSH;    reduced glutathione HNA;    4-hydroxy-2;    3-trans-nonenoic (4-hydroxynonenoic acid);    HNE;    4-hydroxy-2;    3-trans-nonenal (4-hydroxynonenal);    HPLC;    high-performance liquid chromatography;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(94)00922-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytosolic lipid peroxidation product 4-hydroxynonenal (HNE) is rapidly metabolized in mitochondria isolated from rat kidney cortex. About 80% of HNE was degraded within 3 min of incubation. Main products of HNE which were identified in mitochondria were the hydroxynonenoic acid, the 1,4-dihydroxynonene and the glutathione-HNE-conjugate. Furthermore, formation of metabolites of the tricarboxylic acid cycle from HNE is suggested. The quantitative share of HNE binding to proteins was high with about 8% of total HNE consumption after 3 min of incubation. Therefore, rapid degradation of HNE by mitochondria might be involved in an intracellular antioxidative defense system.

【 授权许可】

Unknown