Iron released from transferrin at acidic pH can catalyse the oxidation of low density lipoprotein

FEBS Letters

Leake, David S.¹  Lamb, David J.¹ 

[1] Department of Biochemistry and Physiology, School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading, Berks., RG6 2 AJ, UK

关键词: Acidic pH;    Atherosclerosis;    Iron ion;    Low density lipoprotein;    Oxidation;    Transferrin;    DMEM;    Dulbecco's modified Eagle's medium;    LDL;    low density lipoprotein;    MES;    N-morpholinoethanesulphonic acid;    TBARS;    thiobarbituric acid-reactive substances;   

DOI  :  10.1016/0014-5793(94)00903-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low density lipoprotein (LDL) oxidation within the arterial wall may contribute to the disease of atherosclerosis. We have investigated the conditions under which transferrin (the major iron-carrying protein in plasma) may release iron ions to catalyse the oxidation of LDL. Transferrin that had been incubated at pH 5.5 released approximately 10% of its bound iron in 24 h, as measured by ultrafiltration and atomic absorption spectroscopy. Furthermore, transferrin co-incubated with LDL and l-cysteine at pH 5.5 resulted in the oxidation of the LDL as measured by thiobarbituric acid-reactive substances and electrophoretic mobility. This effect was observed at transferrin concentrations as low as 40% of its average plasma concentration. The release of iron from transferrin in atherosclerotic lesions due to a localised acidic pH may help to explain why LDL oxidation occurs in these lesions.

【 授权许可】

Unknown