Overexpression of TGN38/41 leads to mislocalisation of γ‐adaptin

FEBS Letters

Reaves, Barbara¹  Banting, George¹ 

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol BS81TD, UK

关键词: trans-Golgi network;    Retention;    γ-Adaptin;    FACS;    fluorescence activated cell sorter;    BFA;    Brefeldin A;    FITC;    fluorescein isothiocyanate;    MTOC;    Microtubule Organising Centre;    TRITC;    Texas red isothiocyanate;    TGN;    trans-Golgi network;   

DOI  :  10.1016/0014-5793(94)00813-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

TGN38 and TGN41 are isoforms of a monotopic integral membrane protein which recycles between the trans Golgi network (TGN) and the cell surface, but which, at steady state, is predominantly located in the TGN. Full-length and truncated versions of rat TGN38/41 have been expressed in monkey (COS) and human (Heb7a) cells under the control of the heavy metal inducible Metallothionein IIA promoter. This has allowed the regulated expression of TGN38/41 protein constructs to different levels in the transfected cells. These studies show that (i) controlled overexpression of TGN38/41 results in mislocalisation to parts of the endocytic pathway, (ii) a truncated version of TGN38/41, lacking the cytoplasmic domain, remains in the TGN, and (iii) there is a direct or indirect interaction between the cytoplasmic domain of TGN38/41 and γ-adaptin.

【 授权许可】

Unknown