Differentiation of HL‐60 cells is promoted by H‐toxin of Clostridium septicum

FEBS Letters

Noda, Masatoshi¹  Kato, Iwao¹  Morinaga, Naoko¹ 

[1] Second Department of Microbiology, School of Medicine, Chiba University, 1-8-1 Inohana, Chuoku, Chiba 260, Japan

关键词: Differentiation;    HL-60 cell;    Clostridium septicum;    H-toxin;    ADP-ribosylation;   

DOI  :  10.1016/0014-5793(94)00856-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

H-toxin of Clostridium septicum potentiated dimethyl sulfoxide(DMSO)-induced differentiation of human promyelocytic leukemia HL-60 cells which were monitored by nuclear morphology and production of oxidative radicals. But, H-toxin did not induce differentiation of HL-60 cells in the absence of DMSO. These phenomena were not observed by staphylococcal leukocidin, a cytotoxin affecting to HL-60 cells. In HL-60 cells, ADP-ribosylation of 118, 93, 75 and 58 kDa membrane proteins was observed, but the ADP-ribosylation was not detected either in differentiated HL-60 cells by DMSO or in normal polymorphonuclear leukocytes of human. When the membranes of HL-60 cells were incubated with H-toxin, ADP-ribosylation of the membrane proteins was inhibited. Such suppressive effects on ADP-ribosylation were not observed by DMSO or staphylococcal leukocidin. These data suggest that inhibition of the ADP-ribosylation by H-toxin may play an important role in potentiation of DMSO-induced differentiation of HL-60 cells.

【 授权许可】

Unknown