Induction of differentiation of human leukemia cells by inhibitors of myosin light chain kinase

FEBS Letters

Honma, Yoshio²  Makishima, Makoto²  Hattori, Masao²  Hozumi, Motoo²  Motoyoshi, Kazuo¹  Sampi, Kazumi² 

[1] National Defense Medical College, Tokorozawa, Saitama-359, Japan;Saitama Cancer Center, Ina-machi, Saitama-362, Japan

关键词: Differentiation;    Myosin light chain kinase;    ML-9: ML-7: Leukemia cell: U937 cell;    HL-60 cell;    PKC;    protein kinase C;    MLCK;    myosin light chain kinase;    ML-9;    1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1;    4-diazepine hydrochloride;    ML-7;    1-(5-iodonapthalene-1-sull'onyl)-1H-hexahydro-1;    4-diazepine hydrochloride;    H-7;    1-(5-isoquinoline-sulf'onyl)-2-methylpiperazine dihydrochloride;    SC-9;    N-(6-phenyl-hexyl)-5-chloro-1-naphtalencsulfonamide;    W-7;    N-(6-aminohcxyl)-5-chloro-1-naphthalenesulfonamide;    NBT;    Nitroblue tetrazolium;   

DOI  :  10.1016/0014-5793(91)80044-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Inhibitors of myosin light chain kinase, 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexanydro-1,4-diazepine hydrochloride (ML-9) and 1-(5-iodonaph-thalene-1-sulfonyl)-1 H-hexahydro-1,4-diazepine hydrochloride (ML-7), induced Nitroblue tetrazolium reducing activity, lysozyme activity and morphological maturation of human monoblastic U937, THP-1 and promyelocytic HL-60 cells, but not of erythroblastic K562 cells. However, three analogs of ML-9, which are an inhibitor and an activator of protein kinase C, and a calmodulin antagonist, respectively, did not induce differentiation of the cells.

【 授权许可】

Unknown