Oxygen‐derived radicals stimulate renin release of isolated juxtaglomerular cells

FEBS Letters

Wanner, Christoph¹  Herzog, Christel¹  Galle, Jan¹  Schollmeyer, Peter¹ 

[1] Department of Medicine, Division of Nephrology, University Hospital, Hugstetterstr. 55, D-79106 Freiburg, Germany

关键词: Renin;    Oxygen radical;    Superoxide dismutase;    Juxtaglomerular cell;    Catalase;    Hydrogen peroxide;    JG-cells;    juxtaglomerular cells;    SOD;    superoxide dismutase;    XOD;    xanthine oxidase;   

DOI  :  10.1016/0014-5793(94)00853-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We assessed effects of reactive oxygen metabolites on renin release of juxtaglomerular cells (JG-cells) prepared in primary culture from mouse kidneys. Renin activity was measured in culture supernatants and cells. Basal renin release was increased by incubation of JG-cells with xanthine/xanthine oxidase from 26 ± 1% up to 58 ± 3% of total activity. This increase was slightly inhibited by superoxide dismutase, and was eliminated by addition of catalase, implicating H₂O₂ as an intermediate product in the stimulatory cascade. H₂O₂ applied exogeneously dose-dependently stimulated renin release up to 55 ± 2%; this effect was also prevented by catalase. We propose that reactive oxygen metabolites stimulate renin release in isolated JG-cells. This could have important implications in inflammatory kidney diseases.

【 授权许可】

Unknown