Evidence for intracellular generation of angiotensin II in rat juxtaglomerular cells

FEBS Letters

Thibault, Gaétan²  Garcia, Raul³  Mercure, Chantal²  Reudelhuber, Timothy L¹  Ramla, Djamel²  Deschepper, Christian F² 

[1] MRC Multidisciplinary Research Group on Hypertension, Clinical Research Institute of Montreal, 110 Pine Ave. West, Montreal, Que. H2W 1R7, Canada;Laboratory of Experimental Hypertension and Vasoactive Peptides, Clinical Research Institute of Montreal, 110 Pine Ave. West, Montreal, Que. H2W 1R7, Canada

关键词: Angiotensin II;    Renin;    Juxtaglomerular cell;    Angiotensinogen;   

DOI  :  10.1016/S0014-5793(98)00052-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The formation of the vasoactive peptide angiotensin II (AII) is dependent on the sequential action of two enzymes, renin and angiotensin converting enzyme (ACE), on the substrate angiotensinogen. Although the renin-producing cells of the kidney do not express angiotensinogen, they contain large amounts of AII in the same storage granules that contain renin. When renin expression is suppressed in these cells, AII also disappears. In the current study, we have tested whether the renin-associated disappearance of AII in renal juxtaglomerular (JG) cells is due to a renin-dependent down-regulation of granule biosynthesis and whether receptor-mediated internalization of AII could account for its concentration in these cells. Our results support a model whereby AII peptides are generated within JG cells, presumably by a mechanism which involves the action of endogenous renin on internalized, exogenous angiotensinogen.

【 授权许可】

Unknown