Biosynthetic pathway for a new series of gangliosides, GT1aα and GQ1bα

FEBS Letters

Seyama, Yousuke²  Tai, Tadashi¹  Hirabayashi, Yoshio³  Irie, Fumitoshi²  Li, Yu-Teh³  Jwa Hidari, Kazuya I.-P.³ 

[1] Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medical Science, Hon-komagome, Bunkyo-ku, Tokyo 113, Japan;Department of Physiological Chemistry and Nutrition, Faculty of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan;Laboratory for Glyco Cell Biology, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Hirosawa, Wako-shi, Saitama 351-01, Japan

关键词: Ganglioside;    Sialyltransferase;    Ganglioside biosynthesis;    Cholinergic neuron-specific antigen;    The nomenclature used for gangliosides is based on the system of Svennerhorm and Hirabayashi et al. [9;    24]. Cer;    ceramide;    Gal;    galactose;    Glc;    glucose;    GalNAc;    N-acetylgalactosamine;    NeuAc;    N-acetylneuraminic acid;    TLC;    thin layer chromatography;    HPTLC;    high performance thin layer chromatography;   

DOI  :  10.1016/0014-5793(94)00883-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new class of gangliosides, GT1aα and GQ1bα, were initially identified as cholinergic neuron-specific antigens in bovine brain. These gangliosides have in common α2–6 NeuAc linked to the GalNAc residue in the gangliotetraose core structure. In this study, we have determined the biosynthetic pathways of GT1aα and GQ1bα using rat liver Golgi fraction. The results showed that GT1aα and GQ1bα were synthesized from GD1a and GT1b, respectively, by the action of a GalNAc α2–6 sialyltransferase. It was also demonstrated that these two gangliosides were found to exist as extremely minor components in rat liver.

【 授权许可】

Unknown