src‐related protein tyrosine kinases are physically associated with the surface antigen CD36 in human dermal microvascular endothelial cells

FEBS Letters

Dowd, Pauline M.¹  Brickell, Paul M.²  Bull, Helen A.¹ 

[1] Division of Dermatology, Department of Medicine University College London Medical School, The Rayne Institute, 5 University Street, London WC1E 6JJ, UK;Medical Molecular Biology Unit, Department of Molecular Pathology, University College London Medical School, London, UK

关键词: Microvascular endothelium;    CD36;    src-related protein tyrosine kinase;    ECM;    extracellular matrix;    PTK;    protein tyrosine kinase;    HUVEC;    human umbilical vein endothelial cells;    HDMEC;    human dermal microvascular endothelial cells;    PHS;    pooled human serum;    Iscove's DMEM;    Iscove's modification of Dulbecco's modified Eagle's medium;    DTT;    dithiotreitol;    PMSF;    phenyl methyl sulphonyl fluoride;    LB;    lysis buffer;    BSA;    bovine serum albumin;    NRS;    normal rabbit serum;    SDS;    sodium dodecyl sulphate;    RIPA;    radioimmunoprecipitation assay;    TBS;    Tris-buffered saline;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    BCIP;    bromo-4-chloro-3-indoyl phosphate;    NBT;    nitroblue tetrazolium;   

DOI  :  10.1016/0014-5793(94)00814-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

src-related cytoplasmic PTKs are physically and functionally associated with cell surface receptors and are involved in signal transduction. In this paper we report the identification of src-related proteins p59 ^fyn , pp60^c-scr and p62 ^yes in human microvascular endothelial cells cultured from normal human skin and their physical association with the thrombospondin receptor CD36. Such an association represents a potential signalling pathway by which thrombospondin may regulate angiogenesis.

【 授权许可】

Unknown