Cloning and chromosomal localization of the gene coding for human protein kinase CK1

FEBS Letters

Featherstone, Terence¹  Taillon-Miller, Patricia²  Tapia, Claudio²  Gómez, Claudio²  Allende, Catherine C.³  Allende, Jorge E.² 

[1] Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA;Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 7, Chile;Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Chile

关键词: Casein kinase 1;    Chromosome 13;    Protein kinase;    Gene mapping;    Fluorescent in situ hybridization;   

DOI  :  10.1016/0014-5793(94)00679-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone coding for human protein kinase CK1 (casein kinase 1) has been isolated and sequenced demonstrating that it corresponds to a homolog of the CK1α form found in bovine brain. The derived amino acid sequence of the human CK1α is identical to the bovine counterpart except that it contains 12 extra amino acids at the carboxyl end. Using this cDNA sequence and PCR amplification, YAC genomic clones that contain this human CK1α sequence have been isolated. These YACs have been used for fluorescent in situ hybridization in order to localize the human CK1α gene to chromosome 13q13.

【 授权许可】

Unknown