Voltage‐dependent Ca2+ channels in Chinese hamster ovary (CHO) cells

FEBS Letters

Dufy, B.¹  Prevarskaya, N.¹  Vacher, P.¹  Skryma, R.¹ 

[1] Laboratory of Neurophysiology, CNRS URA 1200, University of Bordeaux II, CNRS URA 1200, 146 rue Léo Saignat, 33076 Bordeaux, France

关键词: CHO-cell;    Calcium current;    Calcium channel;   

DOI  :  10.1016/0014-5793(94)00690-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Voltage-dependent Ca²⁺ channels were identified in CHO-K1 cells, currently used in molecular biology studies. Experimental data obtained at both macroscopic and single-channel levels using the patch-clamp technique show that the Ca²⁺ current in CHO cells is similar to the high-threshold L-type of Ca²⁺ current previously observed in excitable cells. It can be carried by Ca²⁺ or Ba²⁺ ions, blocked by both inorganic (Co²⁺) and organic (nifedipine, isradipine) Ca²⁺ channel blockers. The unitary Ca²⁺ channel activity was characterized by a conductance of 19 pS in 60 mM Ca²⁺, by single exponential distribution of open times (t = 0.84 ms) and biexponential distribution of closed times (t _f, = 1.67 ms, t _s = 7.9 ms ). However, the functional role of these Ca²⁺ channels in CHO cells remains unclear.

【 授权许可】

Unknown