Independent control of transcription initiations from two sites by an initiator‐like element and TATA box in the human c‐erb B‐2 promoter

FEBS Letters

Sawazaki, Tetsuya¹  Mizuguchi, Gaku¹  Roeder, Robert G.²  Ishii, Shunsuke¹  Yamamoto, Tadashi³  Horikoshi, Masami²  Kanei-Ishii, Chie¹ 

[1] Laboratory of Molecular Genetics, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan;Laboratory of Biochemistry and Molecular Biology, The Rockfeller University, New York, NY 10021, USA;Department of Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108, Japan

关键词: c-erbB-2 proto-oncogene;    Transcriptional initiation;    Initiator;    TATA box;   

DOI  :  10.1016/0014-5793(94)00578-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transcription of the human c-erbB-2-proto-oncogene starts mainly at two sites, nucleotide positions + 1 and −69. The present studies have identified an initiator-like element that specifies the position of transcription initiation at position −69. This initiator-like element contains six GGA repeats and is located just downstream from the transcription start site between positions −68 and −45. In addition, both in vitro and in vivo studies indicated that transcription initiation at position +1 is specified by a TATA box 25 bp upstream from the transcription startpoint. Thus, initiation at two sites in the c-erbB-2 promoter is controlled independently by the initiator-like element and the TATA box.

【 授权许可】

Unknown