Cloning and expression of a novel form of leukotriene B4 ω‐hydroxylase from human liver

FEBS Letters

Yamamoto, Satoru¹  Kusunose, Emi²  Kinoshita, Hiroaki³  Kondo, Tetsuaki¹  Kikuta, Yasushi¹  Kusunose, Masamichi¹ 

[1] Department of Food Science and Technology, Faculty of Engineering, Fukuyama University, Fukuyama, Hiroshima 729-02, Japan;Toneyama Institute for Tuberculosis Research, Osaka City University Medical School, Toyonaka, Osaka 560, Japan;2nd Department of Surgery, Osaka City University Medical School, Abeno, Osaka 545, Japan

关键词: Cytochrome P-450;    Leukotriene B4;    ω-Hydroxylase;    cDNA cloning;    Human liver;    P-450;    cytochrome P-450;    LTB4;    leukotriene B4;    PMN;    polymorphonuclear leukocytes;    bp;    base pair(s). CYP refers to a cytochrome P-450 gene(s) and cDNA(s);    which is the nomenclature recommended by Nelson et al. [9];   

DOI  :  10.1016/0014-5793(94)00587-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated and sequenced a cDNA for human liver LTB₄ ω-hydroxylase. The cDNA encoded a protein of 520 amino acids with a molecular weight of 59,853 Da. The cDNA-deduced amino acid sequence showed 87.3% homology to that of human polymorphonuelear leukocytes (PMN) LTB₄ ω-hydroxylase (CYP4F3). Northern blot analysis revealed that the mRNA hybridized to the specific cDNA fragment is expressed in human liver, but not in human PMN. The microsomes from yeast cells transfected with the cDNA catalysed the ω-hydroxylation of LTB₄ with a K _m of 44.8 μM. These results clearly show that a new form of the CYP4F LTB₄ ω-hydroxylase exists in human liver.

【 授权许可】

Unknown