Molecular Dissection of the Interaction between hBLT2 and the G Protein Alpha Subunits

Bulletin of the Korean chemical society

Cheolju Lee¹  Ick Young Kim¹  Krishna Moorthy Vukoti¹  Ho-Jun Kim¹  Won-Kyu Lee¹  Yeon Gyu Yu¹  Eun Gyeong Yang¹ 

关键词: Leukotriene B4;    hBLT2;    G-protein;    Intracellular loop;    GPCR-G protein interaction;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G�? protein including G�? i1, G�? i2, G�? i3, G�? s1, G�? o1, and G�? z. Among the tested G�? subunits, G�? i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 �? 10^?6 M. These results suggest that G�? i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G�? i3.

【 授权许可】

Unknown