Hyaluronate depolymerization activity induced by progesterone in cultured fibroblasts derived from human uterine cervix

FEBS Letters

Saito, Yoshiharu¹  Nakamura, Toshiya²  Tanaka, Kanji²  Endo, Masahiko²  Higuchi, Tsuyoshi²  Takagaki, Keiichi²  Ikeya, Hitoshi²  Morikawa, Akiko²  Tanaka, Akihiro² 

[1] Obstetrics and Gynecology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036, Japan;Departments of Biochemistry and Obstetrics and Gynecology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036, Japan

关键词: Hyaluronate depolymerization;    Progesterone;    Human uterine cervix;    Cultured fibroblast;    Dex;    dexamethasone;    DHAS;    dehydroepiandrosterone sulfate;    PGF2a;    prostaglandin F2a;    PGE2;    prostaglandin E2;    IL-1α;    interleukin 1α;    DMEM;    Dulbecco's modified Eagle medium;    CMF-PBS;    Dulbecco's calcium- and magnesium-free phosphate-buffered saline;    FBS;    fetal bovine serum;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(94)00517-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

High-molecular-weight [¹⁴C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated. [¹⁴C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (M _r about 40,000), whereas that of cervix fibroblasts was not depolymerized, irrespective of incubation period. However, when progesterone was added to the medium of cervix fibroblasts, hyaluronate was depolymerized to the same extent as that in skin fibroblasts. The reducing terminal sugar of the depolymerized hyaluronate was N-acetylglucosamine. These results suggest that a hyaluronate-depolymerizing enzyme, endo-β-N-acetylglucosaminidase, was induced by progesterone in cultured fibroblasts derived from human uterine cervix.

【 授权许可】

Unknown