Urinary trypsin inhibitor suppresses the production of interstitial procollagenase/proMMP‐1 and prostromelysin 1/proMMP‐3 in human uterine cervical fibroblasts and chorionic cells

FEBS Letters

Mori, Yo²  Terao, Toshihiko¹  Imada, Keisuke²  Kanayama, Naohiro¹  Ito, Akira² 

[1] Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 331-31, Japan;Department of Biochemistry, Tokyo University of Pharmacy and Life Science, School of Pharmacy, 1432 Horinouch, Hachioji, Tokyo 192-03, Japan

关键词: Urinary trypsin inhibitor (UTI);    Matrix metalloproteinase;    Collagenase;    Stromelysin 1;    Premature rupture of fetal membrane;    Human uterine cervix;    Human fetal membrane;    MMP;    matrix metalloproteinase;    TIMP;    tissue inhibitor of metalloproteinases;    UTI;    urinary trypsin inhibitor;    PROM;    premature rupture of fetal membrane;    FBS;    fetal bovine serum;    DMEM;    Dulbecco's modified Eagle's medium;    MEM;    Eagle's minimum essential medium;    IL;    interleukin;    SDS-PAGE;    sodium dodesyl sulfate-polyacrylamide gel electrophoresis;    LAH;    lactalbumin hydrolysate;    GAPDH;    glyceroaldehyde 3-phosphate dehydrogenase;    SSC;    standard saline citrate;   

DOI  :  10.1016/S0014-5793(97)01319-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanisms by which urinary trypsin inhibitor (UTI) prevents preterm premature rupture of fetal membrane and premature cervical ripening were investigated. We, therefore, examined the effects of UTI on the production of matrix metalloproteinases (MMPs) which closely participate in the breakdown of extracellular matrix in cultured human uterine cervical fibroblasts and human chorionic cells. UTI suppressed specifically the production of interstitial procollagenase/proMMP-1 and prostromelysin 1/proMMP-3 from both cells in a dose-dependent manner (0.32-1.28 μM). This suppression was accompanied by a decrease in steady-state levels of their mRNAs. These results indicate for the first time that UTI down-regulates the production of proMMP-1 and proMMP-3 accompanying with the decrease in the expression of their mRNAs, and therefore UTI actually participates in the maintenance of fetal membranes and/or uterine cervix by overall suppression of MMP production along with the known inhibitory actions towards serine proteinases.

【 授权许可】

Unknown