Identification of two distinct synucleins from human brain

FEBS Letters

Spillantini, Maria Grazia¹  Goedert, Michel¹  Jakes, Ross¹ 

[1] MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

关键词: α-Synuclein;    β-Synuclein;    Precursor of the non-Aβ component;    Phosphoneuroprotein 14;    Alzheimer's disease;    PHF;    paired helical filament;    NACP;    non-Aβ component of Alzheimer's disease precursor;    PNP14;    phosphoneuroprotein 14;   

DOI  :  10.1016/0014-5793(94)00395-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two abundant proteins of 140 and 134 amino acids were purified and sequenced from human brain. They were identified through their reactivity on immunoblots with a partially characterised monoclonal antibody that recognises tau protein in a phosphorylation-dependent manner. The 140 amino acid protein is identical with the precursor of the non-Aβ component of Alzheimer's disease amyloid which in turn is highly homologous to synuclein from Torpedo electroplaques and rat brain. The 134 amino acid protein is the human homologue of bovine phosphoneuroprotein 14; it is 61% identical in sequence to the 140 amino acid protein. The previously unrecognised homology between these two proteins defines a family of human brain synucleins. We refer to the 140 and 134 amino acid proteins as α-synuclein and β-synuclein, respectively. Both synucleins are expressed predominantly in brain, where they are concentrated in presynaptic nerve terminals.

【 授权许可】

Unknown