The Pseudomonas phytotoxin coronatine mimics octadecanoid signalling molecules of higher plants

FEBS Letters

Niesel, U.²  Brodschelm, W.⁴  Bublitz, F.¹  Kutchan, T.M.³  Gorba, T.²  Weiler, E.W.² 

[1] Lehrstuhl für Mikrobiologie, Friedrich-Schiller-Universität, D-07743 Jena, Germany;Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, D-44780 Bochum, Germany;Laboratorium für Molekulare Biologie Ludwig-Maximilians-Universität, D-80333 München, Germany;Lehrstuhl für Pharmazeutische Biologie, Ludwig-Maximilians-Universität, D-80333 München, Germany

关键词: Coronatine;    Jasmonic acid;    Methyljasmonate;    12-Oxo-phytodienoic acid;    Coronatine (mode of action);   

DOI  :  10.1016/0014-5793(94)00411-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phytotoxic principle, coronatine, which is present in several pathovars of the plant pathogen, Pseudomonas syringae was shown to be highly active in completely different, jasmonate-selective bioassays. At nanomolar to micromolar concentrations, coronatine induced the accumulation of defense-related secondary metabolites in several plant cell cultures, induced transcript accumulation of the elicitor-responsive gene encoding the berberine bridge enzyme of Eschscholtzia californica, as well as the coiling response of Bryonia dioica tendrils. Biological activity critically depended upon the structure of coronatine, and slight modifications, such as methylation of the carboxyl moiety or reduction of the carbonyl group, rendered the molecules almost inactive. Coronafacic acid, obtained by hydrolysis of coronatine, was also nearly inactive. Coronatine did not elicit the accumulation of endogenous jasmonic acid in the systems analyzed. While coronafacic acid is similar in structure to jasmonic acid, we found coronatine to be a close structural analogue of the cyclic C18-precursor of jasmonic acid, 12-oxo-phytodienoic acid. The phytotoxic symptoms produced by coronatine can now be understood on the basis of the toxin's action as a mimic of the octadecanoid signalling molecules of higher plants.

【 授权许可】

Unknown