Geraniol 10‐hydroxylase1, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis

FEBS Letters

Peltenburg-Looman, Anja M.G.¹  Memelink, Johan²  Unver, Nehir¹  Collu, Graziella¹  Verpoorte, Robert¹  van der Heijden, Robert¹ 

[1] Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Leiden University, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA Leiden, The Netherlands;Institute of Molecular Plant Sciences, Clusius Laboratory, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

关键词: Alkaloid;    Cytochrome P450;    Geraniol 10-hydroxylase;    Indole alkaloid;    Methyljasmonate;    Secondary metabolism;    Terpenoid indole alkaloid;    Catharanthus roseus;    CaMV;    cauliflower mosaic virus;    CPR;    cytochrome P450 reductase;    GC/MS;    gas chromatography/mass spectrometry;    G10H;    geraniol 10-hydroxylase;    MeJA;    methyljasmonic acid;    P450;    cytochrome P450 monooxygenase;    ORF;    open reading frame;    PCR;    polymerase chain reaction;    Rps9;    ribosomal protein S9;    Sgd;    strictosidine β-D-glucosidase;    Sls;    secologanin synthase;    Str;    strictosidine synthase;    Tdc;    tryptophan decarboxylase;    TIA;    terpenoid indole alkaloid;   

DOI  :  10.1016/S0014-5793(01)03045-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Geraniol 10-hydroxylase (G10H) is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. Catharanthus roseus (Madagascar periwinkle) contains monoterpenoid indole alkaloids, several of which are pharmaceutically important. Vinblastine and vincristine, for example, find widespread use as anti-cancer drugs. G10H is thought to play a key regulatory role in terpenoid indole alkaloid biosynthesis. We purified G10H from C. roseus cells. Using degenerate PCR primers based on amino acid sequence information we cloned the corresponding cDNA. The encoded CYP76B6 protein has G10H activity when expressed in C. roseus and yeast cells. The stress hormone methyljasmonate strongly induced G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in a C. roseus cell culture.

【 授权许可】

Unknown