A mutation blocking the formation of membrane or periplasmic endogenous and exogenous c‐type cytochromes in Escherichia coli permits the cytoplasmic formation of Hydrogenobacter thermophilus holo cytochrome c 552

FEBS Letters

Crooke, Helen²  Cole, Jeff A.²  Ferguson, Stuart J.¹  Sambongi, Yoshihiro¹ 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK;School of Biochemistry, University of Birmingham, Birmingham, B15 2TT, UK

关键词: Bacterial c-type cytochrome biogenesis;    Escherichia coli;    Exogenous c-type cytochrome expression;   

DOI  :  10.1016/0014-5793(94)00399-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A mutant of Escherichia coli, JCB606, shown to be pleiotropically deficient in the formation of endogenous membrane and periplasmic c-type cytochromes, synthesised the apo form of the erogenous cytochrome c ₅₅₀ from Paracoccus denitrificans, but not the holo form. In contrast, a cytoplasmically located holo form of Hydrogenobacter thermophilus cytochrome c ₅₅₂ was found in E. coli JCB606. These findings support the proposition that the formation of the cytoplasmic H. thermophilus cytochrome c ₅₅₂ in E. coli does not involve the physiological pathway of c-type cytochrome biosynthesis in E. coli and that the haem insertion may be uncatalysed.

【 授权许可】

Unknown