Synthesis of holo Paracoccus denitrificans cytochrome c 550 requires targeting to the periplasm whereas that of holo Hydrogenobacter thermophilus cytochrome c 552 does not

FEBS Letters

Ferguson, Stuart J.¹  Sambongi, Yoshihiro¹ 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

关键词: Bacterial c-type cytochrome biogenesis;    Heme attachment;    Periplasm;    Signal sequence;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(94)80174-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression from a plasmid of the complete gene, including the codons for the N-terminal periplasmic targeting signal, for cytochrome c ₅₅₀ of Paracoccus denitrificans led to the formation of the holo protein in the periplasms of both P. denitrificans and Escherichia coli. Expression of the gene from which the region coding for the targeting signal had been specifically deleted resulted in formation of apo-protein in the cytoplasms of both organisms. These findings are consistent with haem attachment occurring in the periplasm. In contrast, the formation of holo cytochrome c ₅₅₂ from Hydrogenobacter thermophilus following expression of the gene lacking the periplasmic targeting sequence in either P. denitrificans or E. coli is attributed to spontaneous cytoplasmic attachment of haem to the thermostable protein.

【 授权许可】

Unknown