Fatty acid binding site of the mitochondrial uncoupling protein

FEBS Letters

Freisleben, Hans-Joachim¹  Ježek, Petr² 

[1] Gustav-Embden-Zentrum der Biologischen Chemie, J.W. Goethe-Universität, Theodor-Stern-Kai 7, D-60 596 Frankfurt am Main, Germany;Institute of Physiology, Department of Membrane Transport Biophysics, Academy of Sciences of the Czech Republic, Videñská 1083, CZ-14220 Prague, Czech Republic

关键词: Uncoupling protein;    Brown fat mitochondrion;    H+ transport;    Reconstitution;    Fatty acid binding site;    5-DOXYL-stearic acid;    BAT;    brown adipose tissue;    h +1;    low field peak;    h −1 high field peak;    h 0/h −1;    the ratio of heights of the middle- and high-field peak;    Octyl-POE;    octyl-pentaoxyethylene;    PBFI;    potassium-binding benzofuran phtalate;    16-SASL;    16-DOXYL-stearic acid;    5-SASL;    5-DOXYL-stearic acid;    where DOXYL is the 4;    4-dimethyl-3-oxazolinyloxy residue;    τR;    rotational correlation time;    TEA;    tetraethyl-ammonium;    TES;    N-tris [hydroxymethyl]methyl-2-aminoethane sulfonic acid;    UcP;    uncoupling protein;    w(1/2)h+1);    half height peak width;   

DOI  :  10.1016/0014-5793(94)80599-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fatty acid binding site on isolated mitochondrial uncoupling protein (UcP) is demonstrated using EPR spectroscopy of 5-DOXYL-stearic acid (5-SASL), which also activated H⁺ transport in proteoliposomes containing UcP. In the presence of UcP the EPR spectrum showed reproducible broadening of the low field peak as well as an increase in h _+1I/h _+1M ratio, rotational correlation time and in order parameter. The half-height width of the low field peak was even doubled in the presence of another UcP ligand, GDP. Palmitic acid reversed the effect of 5-SASL and non-ionizable 5-DOXYL-decane did not exhibit it.

【 授权许可】

Unknown