1 kb of the lactase‐phlorizin hydrolase promoter directs post‐weaning decline and small intestinal‐specific expression in transgenic mice

FEBS Letters

Olsen, Jørgen¹  Hansen, Gert H.¹  Prydz, Hans²  Spodsberg, Nikolaj¹  Mehlum, Anja²  Troelsen, Jesper T.¹  Norén, Ove¹  Sjöström, Hans¹ 

[1] Department of Medical Biochemistry and Genetics, Biochemistry Laboratory C, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark;Biotechnology Center of Oslo and Animal Department, The University Clinic, University of Oslo, Blindern, N-0316 Oslo 3, Norway

关键词: Lactase-phlorizin hydrolase;    Tissue specificity;    Small intestine;    Enterocyte;    Hypolactasia;    Lactose intolerance;   

DOI  :  10.1016/0014-5793(94)80519-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch −17 to −994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit β-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

【 授权许可】

Unknown