In vitro binding of the two‐finger repressor CreA to several consensus and non‐consensus sites at the ipnA upstream region is context dependent

FEBS Letters

Espeso, Eduardo A.¹  Peñalva, Miguel A.¹ 

[1] Centro de Investigacione Biológicas del CSIC, Velázquez 144, Madrid, 28008, Spain

关键词: Transcription factor;    DNA binding;    Zinc finger;    Ascomycetes;    Carbon catabolite repression;   

DOI  :  10.1016/0014-5793(94)80581-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The two zinc-fingers of the Aspergillus nidulans repressor CreA recognize the consensus hexanucleotide 5'-SYGGRG-3'. We have determined all the CreA binding sites in a ∼2 kb region upstream the ipnA gene. Our analysis shows that (i) CreA binds to certain consensus sites in a context-dependent manner; (ii) five non-consensus 6-bp sequences are also recognized by CreA; this non-canonical binding correlates with the presence of a second, neighbouring CreA binding site, suggesting that recognition of two linked sites stabilizes CreA binding. Our results suggest that the binding possibilities of CreA might be more complex than originally envisaged.

【 授权许可】

Unknown