FEBS Letters | |
In vitro binding of the two‐finger repressor CreA to several consensus and non‐consensus sites at the ipnA upstream region is context dependent | |
Espeso, Eduardo A.1  Peñalva, Miguel A.1  | |
[1] Centro de Investigacione Biológicas del CSIC, Velázquez 144, Madrid, 28008, Spain | |
关键词: Transcription factor; DNA binding; Zinc finger; Ascomycetes; Carbon catabolite repression; | |
DOI : 10.1016/0014-5793(94)80581-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The two zinc-fingers of the Aspergillus nidulans repressor CreA recognize the consensus hexanucleotide 5'-SYGGRG-3'. We have determined all the CreA binding sites in a ∼2 kb region upstream the ipnA gene. Our analysis shows that (i) CreA binds to certain consensus sites in a context-dependent manner; (ii) five non-consensus 6-bp sequences are also recognized by CreA; this non-canonical binding correlates with the presence of a second, neighbouring CreA binding site, suggesting that recognition of two linked sites stabilizes CreA binding. Our results suggest that the binding possibilities of CreA might be more complex than originally envisaged.
【 授权许可】
Unknown
【 预 览 】
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