Determination of the DNA binding site of the GAL4 protein A photo‐CIDNP study

FEBS Letters

Shirakawa, Masahiro¹  Serikawa, Yasuharu¹  Kyogoku, Yoshimasa¹ 

[1] Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan

关键词: NMR;    Photo-CIDNP;    GAL4;    UASG;    Zinc finger;    DNA binding;   

DOI  :  10.1016/0014-5793(92)80115-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

After the assignment of ¹H NMR signals of aromatic side chains by means of specific deuteration, we analyzed the DNA binding site of GAL4 by measuring photo-CIDNP spectra. The results showed that Trp³⁶ is involved in both the specific interaction with UAS_G and non-specific DNA binding. This residue is located inside the Cys-rich region, but outside the putative Zn-finger. The photo-CIDNP spectrum also showed that the side chains of Tyr⁴⁰ and His⁵³ are not exposed on the surface of the protein.

【 授权许可】

Unknown