Staphylococcal nuclease and its complexes with nucleotidic inhibitors

FEBS Letters

Cozzone, Patrick J.²  Kaptein, Robert¹ 

[1] Physical Chemistry Laboratory, University of Groningen, Nijenborgh 16, 9747 AG Groningen, The Netherlands;Laboratoire de Biologie Physicochimique, Université de Provence, Place V. Hugo 13003, Marseille, France

关键词: Staphylococcal nuclease;    NMR;    Photo-CIDNP;    Phosphonates;    Interaction;   

DOI  :  10.1016/0014-5793(83)80208-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A laser photo-CIDNP study at 360 MHz of Staphylococcal nuclease shows that calcium binding induces a switch in the accessibility of Tyr₈₅ and Tyr₁₁₅. This switch, which can be reverted by subsequent addition of pdTp inhibitor, might be relevant to the induction of nucleolytic activity upon calcium binding. The conformations of the ternary complexes of calcium (containing nuclease with pdTp, dTpCH₂dTp and dTpCH₂dTpCH₂dT) are very similar and are interpreted in terms of comparable binding patterns at the active site of the enzyme.

【 授权许可】

Unknown