Expression of α‐1,3 linkage‐containing oligomannosyl residues in a cell‐wall mannan of Candida tropicalis grown in yeast extract‐Sabouraud liquid medium under acidic conditions

FEBS Letters

Kobayashi, Hidemitsu²  Takahashi, Shin-ichi²  Suzuki, Shigeo²  Matsuda, Kyoko²  Shibata, Nobuyuki²  Hisamichi, Kanehiko¹  Suzuki, Masako²  Suzuki, Akifumi² 

[1] First Department of Medicinal Chemistry, Tohoku College of Pharmacy, 4-4-1 Komatsushima, Sendai Aoba-ku, Miyagi 981, Japan;Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, 4-4-1 Komatsushima, Sendai Aoba-ku, Miyagi 981, Japan

关键词: Yeast cell-wall mannan;    Chemical structure;    Nuclear magnetic resonance;    α-1;    3 Linkage;    β-1;    2 Linkage;    Candida tropicalis;   

DOI  :  10.1016/0014-5793(94)80576-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the cell-wall mannan obtained from Candida tropicalis IFO 1647 strain cells grown in yeast extract-Sabouraud medium at pH 3.0 by two-dimensional homonuclear Hartmann-Hahn spectroscopy. The results indicate that the phosphate group and the side chains containing a β-1,2-linked mannopyranose unit decreased compared to those of mannan from cells grown under conventional conditions (pH 5.9) with concomitant expression of α-1,3 linkage-containing oligomannosyl side chains. The results of acetolysis of these mannans indicated that the presence of α-1,3-linked mannopyranose unit existed in side chains corresponding to pentaose and hexaose, Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, and Manpα1-2Manpα1-3Manpα1-2Manpα1-2Manpα1-2Man, in the mannan from cells grown at pH 3.0.

【 授权许可】

Unknown